Objective:To investigate the effects of Shugan-liangxue prescription(SGLX) from traditional Chinese medicine combined with anastrozole(ANA) on the serum estradiol(E2) level and bone metabolism in ovariectomized(OVX) Sprague-Dawley(SD) rats.Methods:The postmenopausal animal model was established after OVX and testified by vaginal smear method.Six-month SD rats were randomly divided into model

SGLX

ANA

ANA+SGLX

alendronate sodium(AL) and sham groups.After 16 weeks intragastric administration

the bone mineral density(BMD) of lumbar vertebra and femur were measured by dual energy X-ray absorptiometry.The serum E2

bone gla protein(BGP) and prostaglandin E2(PGE2) were detected by radioimmunoassay and bone alkaline phosphatase(BAP)

carboxyterminal propeptide of type Ⅰprocollagen(PICP)

pyridinoline(PYD)

tartrate-resistant acid phosphatase(TRAP)and cross-linked carboxyterminal telopeptide of typeⅠcollagen(ICTP) were determined by ELISA.Results: Serum E2 level decreased in SGLX

ANA and ANA+ SGLX groups compared with the model group(P<0.05

P<0.01

P<0.001)

BMD had no significant difference compared with the model.In ANA+SGLX group

serum BGP level increased

PYD and PGE2 declined significantly compared with ANA group(P<0.05

P<0.05

P<0.01).PYD decreased greatly in SGLX and AL groups compared with the model(P<0.001

P<0.001).Conclusions: This study showed that SGLX combined with ANA decreased the serum E2 level

meanwhile had the effect to relieve the side reaction of bone induced by ANA.