The Caco-2 cell model is an in vitro model for screening drug oral absorption characteristics. It has been used widely for evaluating drug absorption characteristics and transportation mechanism.The authors describ the Caco-2 cells culture and how to establish and verify the cell monolayer model.The mechanism of its application in the drug absorption and transportation study is investigated.The applications for passive transportation of Chinese material medica

vector mediated active transportation

efflux mechanism and the drug-drug interaction in the absorption process are discussed in detail.The efficiency on the absorption and transportation mechanism research of Chinese material medica for the Caco-2 cell is evaluated.Finally

the prospect of the cell model in future application is put forward.