Objective:To develop a RRLC method for the content determination of flavonoids in Fructus Aurantii Immaturus. Method: RRLC was applied with a Zobax Extend-C18 colunmn by methanol-2% ethanoic acid (33∶77) as the mobile phase.The flow rate was 1.0 mL·min-1 .The column temperature was 30℃ and the UV detection wavelength was 283 nm. Result: In the 27 batches samples

19 batches contained hesperidin

narirutin

neohesperidin and naringin

and they can divide into two groups by the ratio of flavonoids: in one group the content of hesperidin and narirutin were higher

and in another group the content of neohesperidin and naringin were higher; The other 8 batches only contain hesperidin and narirutin

and the content of hesperidin was higher. The content range of 4 kinds of main flavonoids in each sample were from 12.4% to 38.07%. Conclusion: The method is stable and reliable

and the analysis time of RRLC method was one-third of that of the HPLC method. The types and the content ratios of flavonoids in Fructus Aurantii Immaturus were different.