LI Ming, CHEN Zhi-wei, LI Yu-cui, et al. Determination of Baicalin and Paeoniflorin in Yangkun Pills by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(4): 12-14.
LI Ming, CHEN Zhi-wei, LI Yu-cui, et al. Determination of Baicalin and Paeoniflorin in Yangkun Pills by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(4): 12-14.DOI:
Objective: To develop an RP-HPLC method for the determination of baicalin and paeoniflorin in Yangkun Pills.Method: The sample was separated at 30 ℃ on a Phenomenex Luna C18column(250 mm×4.6 mm
5 μm) eluted with acetonitrile and water containing 0.2% Phosphoric acid in gradient mode.The detection wavelength was 230 nm
and the flow rate was 1.0 mL·min-1.Result: Two compounds were baseline-isolated
the linear relationships of the injection amounts and peak areas for both baicalin and paeoniflorin were excellent.Conclusion: The method was simple
rapid
precise and repeatable.This method can be used for controlling the quality of Yangkun Pills.