HPLC法测定古方定志丸不同配伍中的远志皂苷元含量

房敏峰; 陈焕; 付志玲; 孟雪; 郑晓晖

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HPLC法测定古方定志丸不同配伍中的远志皂苷元含量

更新时间：2024-01-04

- HPLC法测定古方定志丸不同配伍中的远志皂苷元含量
- Determination of Senegenin Contents in Traditional Formula Dingzhi Pill and its Different Compatibility of Recipes by HPLC
- 中国实验方剂学杂志 2009年15卷第4期页码：10-11
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- 基金信息：
  
  陕西省社发攻关项目(2008K16-03);陕西省教育厅产业化培育项目(07JC17)
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- 纸质出版日期：2009
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房敏峰, 陈焕, 付志玲, 等. HPLC法测定古方定志丸不同配伍中的远志皂苷元含量[J]. 中国实验方剂学杂志, 2009,15(4):10-11.

FANG Min-feng, CHEN Huan, FU Zhi-ling, et al. Determination of Senegenin Contents in Traditional Formula Dingzhi Pill and its Different Compatibility of Recipes by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(4): 10-11.
房敏峰, 陈焕, 付志玲, 等. HPLC法测定古方定志丸不同配伍中的远志皂苷元含量[J]. 中国实验方剂学杂志, 2009,15(4):10-11. DOI：

FANG Min-feng, CHEN Huan, FU Zhi-ling, et al. Determination of Senegenin Contents in Traditional Formula Dingzhi Pill and its Different Compatibility of Recipes by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2009, 15(4): 10-11. DOI：

摘要

目的:测定古方定志丸不同配伍中远志皂苷元的含量。方法:采用高效液相色谱法。色谱柱:SHIMADZUC18(4.6mm×150mm

5μm);流动相:乙腈-0.05%磷酸水溶液(41∶59);检测波长:205nm;流速:1.0mL·min-1。结果:定志丸全方组、远志-人参组、远志-石菖蒲组、远志-茯苓组、远志-朱砂组、远志-蜂蜜组和远志单味组中远志皂苷元含量分别为0.359%、0.397%、0.313%、0.294%、0.563%、0.466%和0.449%。结论:方中不同药味与远志配伍后

对远志皂苷元的含量有不同程度的影响。

Abstract

Objective:To determine the contents of dissolved senegenin in Dingzhi pill and its compatible recipes.Methods: Senegenin contents both in Dingzhi pill and its compatible recipes were determined by HPLC

which are compared with senegenin contrast from national institute for the control of pharmaceutical and biological products(NICPBP).Results: The contents of dissolved senegenin was 0.359% in DingZhi pill group

0.397% in Yuan-Ren group

0.313% in Yuan-Shi group

0.294% in Yuan-Fu group

0.563% in Yuan-Zhu group 0.466% in Yuan-Feng group and 0.449% in single Radix Polygara.Conclusion: Radix Polygara combined with different herbs in Dingzhi pill would evidently affect the contents of dissolved Senegenin.

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