Objective:To observe the effects of the method of soothing the liver and strengthening the spleen on gastrointestinal hormone somatostain(SS) and substance P(SP) in animals model of irritable bowel syndrome(IBS).Methods:32 Wistar rats were divided randomly into normal control group

model group

Tongxieyaofang group and dicetel group.The rat model of irritable bowel syndrome(IBS) was made by binding stress with taking senna decoction.Changes of diarrhea rate were observed in these groups.Radioimmunoassay was used to detect the level of SS and SP in plasma and intestinal tissue in rats of each group.Results:The diarrhea rates in Tongxieyaofang group were decreased after treatment

and had the statistics significance in comparison with model group.The contents of SS and SP in plasma and intestinal tissue in model group were higher than those in normal group(P<0.05

P<0.01).The levels of plasma and intestinal tissue SS and SP in Tongxieyaofang group were markedly decreased than those in model group(P<0.05).Conclusions:The method of soothing the liver and strengthening the spleen had the effect of regulating gastrointestinal hormones and reducing the levels of SS and SP in animal model of IBS.