Objective:To explore the method of isolation

cultivation and identification of rat vascular adventitial fibroblasts in vitro

and observe its biological behaviour.Methods:Dulbecco's modified Eagle's medium(DMEM) was the basic culture medium

being supplemented with 20% fetal bovine serum using for culturing thoracic aortal adventitia tissue pieces

and with 10% fetal bovine serum using for culturing passage cells.The fibroblasts were incubate at 37 ℃ in a humidified atmosphere of 5%CO2 and 95% air in the incubator.Growth kinetics

cellular morphologies were observed

and the cultured cells were identified by cell cytoskeleton immunostaining.Results: The isolated fibroblasts could grow and proliferate in vitro

and immunostaining of vimentin was positive

while which of desmin and vWF were negative.The exponential phase of growth was from the second to the fourth day after passage.Conclusion: Rat vascular adventitial fibroblasts can be cultured in stable condition in vitro

and sufficient and reliable target cells can be obtained for the study of mechanisms of vascular stenosis at molecular level.