ZHU Yin-long, CHEN Ping, ZHANG Ya-huan. Study on Determination of Polysaccharide in (P.Karst.) Fiasson[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(9): 1-3.
ZHU Yin-long, CHEN Ping, ZHANG Ya-huan. Study on Determination of Polysaccharide in (P.Karst.) Fiasson[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(9): 1-3.DOI:
Objective:To establish UV method to determine polysaccharide in Fomitiporia robusta(P.Karst.) Fiasson.Method:The parameter of colorimetric method was optimiged through L9(34) orthogonal design with the index of glucose
by the use of phenyl hydrate-sulfuric acid
at the 488 nm.Result:The significant effects of phenyl hydrate-sulfuric acid volume on glucose content were discovered.The average of recovery was 96.6% and RSD 1.71% for glucose with in good linear relation in 3.223~12.89 μg·mL-1 with a correlation coefficient of 0.999 1.Conclution: The proposed method is sensitive
rapid and accurate.It can be used to assay polysaccharide with method indican.