Objective:To study the effect of penetration enhancers on the transdermal penetration of peoniflorin in the Liaojin membrares.Methods:We firstly established the experimental method of transdermal penetration

and the methodology study was carried out at the same time.Furthermore

we added 1%Borneol

3%Borneol

8%Borneol

1% Azone

3% Azone and 5% Azone

3% 1

2-Propanediol

5% 1

2-Propanediol

10% 1

2-Propanediol

1% Azone+8%Borneol

3% Azone+8%Borneol

5% Azone+8%Borneol

5% 1

2-Propanediol+8%Borneol

5% 1

2-Propanediol+8%Borneol+3% Azone and 10%1

2-Propanediol+8%Borneol+5% Azone into the membrares.The cumulative penetration amounts and anatonosis multiple were determined during the test of transdermal penetration.Results:The cumulative penetration amounts and anatonosis multiple released from the membrares increased

and the effect to use mix penetration enhancers was far superior compared with penetration enhancers alone.Conclusions:The results indicate that quantitative penetration enhancers can facilitate drug through corneum into dermis

but to use mix penetration enhancers is far superior compared with penetration enhancers alone.