LU Mei1, XIE Dong2, XIAN Yin-li3. RP-HPLC Simultaneous Determination of Geniposide and Baicalin in Lanqin Oral Solution[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(9): 7-8.
LU Mei1, XIE Dong2, XIAN Yin-li3. RP-HPLC Simultaneous Determination of Geniposide and Baicalin in Lanqin Oral Solution[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(9): 7-8.DOI:
Objective:To develop an HPLC method for the determination of geniposide and baicalin in Lanqin oral solution.Methods:Analysis was carried out at 40 ℃ on a Nova-Pak C18 column(3.9 mm×150 mm
4 μm)with methanol-0.1%TFA(25∶75)as the mobile phase
detecting at 280 nm.The results were calculated with the external standard method.Results:The average recovery was 100.8%
RSD=1.6%(n=9)for geniposide
the linear range was 0.50~2.5 μg
r=0.999 9 and the average recovery was 101.2%
RSD=2.0%(n=9)for baicalin
the linear range was 0.21~1.26 μg
r=0.999 4.Conclusion: It can be used for the quality control of Lanqin oral solution.