Objective:To explore further the pathogenesis of acquired aplastic anemia(AA) and the curative effect mechanism of Shengxue Mixture(SXM)

witch is a Chinese materia medica compound with the function of reinforcing the spleen and nourishing the kidney and promoting blood circulation.Methods: Cyclosporin A as a control

mRNA and protein level and activity of NF-κB in bone marrow of mice model for immune-mediated aplastic anemia(AA) were detected with Q-PCR and immunohistochemistry technique before and after given SXM.Result: No significant changes for NF-κB mRNA expression were observed in bone marrow single nuclear cells(SNCs) of mice with AA

but NF-κB/p65 protein were highly expressed.Otherwise

p-IκBα protein lowerly.However

SXM could decrease NF-κB/p65 protein and increase p-IκBα.By comparison

not only NF-κB/p65 but also p-IκBαwas decreased significantly by Cyclosporin A.Conclusion: Abnormal protein expression and activity changes of NF-κB may play a role in mice model for infusion-induced AA.And SXM may improve hematopoiesis function of the mice by modulating expression and activity of NF-κB.