Objective:To study anti-inflammatory effect of Paino powder and quantitative analysis of the composition.The Paino powder is composed of Fructus Aurantii Immaturi(FAI)

Radix Paeoniae Alba(RPA) and Radix Platycodonis(RP).Methods: The animal model of acute inflammation was established in mice by acetic acid with infiltration increasing of capillary vessel.An orthogonal design was used to assign experimental groups of the combinations of components.The groups of aspirin and saline were selected as the controls.Quantitative analysis of the components and their pharmacodynamic interactions were analyzed by the optical density(OD) of infiltrated Evans Blue.Results: Paino powder and the different combinations of components showed obvious anti-inflammatory effect

and the predicted value of OD is 0.067～0.161.The best anti-inflammatory effect is the whole formulation of Paino powder

while comparing with the group of aspirin the difference was insignificant(P>0.05).The interaction of whole formulation of Paino powder showed antagonism

and the contribution to combined effect of each component: FAI>RPA>RP.Conclusion: Paino powder has significantly anti-inflammatory effect

and FAI shows the best effective.The interaction of combinations is antagonism in anti-inflammatory effect.