Objective:To explore the protective mechanism of Chinese Herb-Da Huang on the vascular disease in diabetes mellitus(DM) rats.Methods:The DM rat model was made.Da Huang was given to treatment group.8 weeks later

blood sample was taken to test nitric oxide(NO) and endothelin-1(ET-1).Thoracic aortic rings to observe the ratio of different concentration of Ach inhibite constraction caused by NE.Took down another part of aorta to make pathology slice

method of SP immunohistochemistry staining

observed the expression of ICAM-1 and VCAM-1.Result:Da Huang group obviously decreased plasma ET-1 and increased the level of NO comparied with Diabetes group(P<0.05)

but could not reach the level of the normal control group(P<0.05).The expression of ICAM-1 and VCAM-1 could be obviously inhibited in Da Huang group comparied with diabetes contrast group.But obviously stronger than normal control group(P<0.05).Conclusion:Chinses medicine Da Huang could decrease the level of ET-1 and increase the level of NO in diabetes rats

they have the function of protect the endothelium of vessel

and could inhibte the expression of ICAM-1 and VCAM-1

act as the role of anti-atherosclerosis.