Objective:To analyse the constituents and determine the biological activity of volatile oil from the tubers of Polygonatum cyrtonema.Methods:The volatile oil was obtained by steam distillation.And its chemical constituents were analyzed by GC-MS.Biological activity of the volatile oil was screened with microbiological method and Alamar Blue method.Results:16 compounds were identified

making up 95.97% of the total contents;The volatile oil could inhibit the growth of Escherichia coli、Staphylococcus aureus and Rhodotorula glutinis The volatile oil could obviously inhibit the growth of cultured tumor cells

and the inhibitory rate was 98.08% at the volatile oil concentration of 100 μg·mL-1.Conclusion:The results indicated that the biological activity of volatile oil of Polygonatu cyrtonema was strong

and it provided the scientific basis for further develop ment of Polygonatum cyrtonema.