Objective:To explore the preliminary pharmacological basis of treating different diseases with the same therapeutic principle with Danggui-Shaoyao San(DSS)

and its effect on various behavior changes were observed in ovariectomized(OVX) mice.Methods: The effect of DSS on the motor activity

pain tolerance and sleep condition of OVX mice was observed through the open-field test

hot-plate test and the sleep test induced by pentobarbital at hypnoid dosage respectively.Meanwhile

the hypothalamus gonadotropin-releasing hormone(GnRH) level was measured by radio immunoassay(RIA) and uterus index was calculated.Results: DSS at the dose of 500 mg·kg-1 could remarkably reduce the motor activity of OVX mice by decreasing its ambulation and rearing times

raise the pain threshold to alleviate the pain reaction

improve the sleep condition through shortening the sleep latent period and prolonging the sleep time of OVX mice.But it had no effects on GnRH level in hypothalamus and the uterus index

which was different from the estrogen control.Conclusion: DSS can improve the behavior changes of OVX mice.This might be the preliminary pharmacological basis of treating different diseases with the same therapeutic principle.