黄芪组分配伍对乳鼠肥大心肌细胞肌酸激酶同功酶mRNA表达的影响

苏敬泽; 农一兵; 温志浩; 林谦

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黄芪组分配伍对乳鼠肥大心肌细胞肌酸激酶同功酶mRNA表达的影响

更新时间：2024-01-04

- 黄芪组分配伍对乳鼠肥大心肌细胞肌酸激酶同功酶mRNA表达的影响
- Radix Astragali Affect mRNA Expression of Isoenzyme Creatine Kinase of Cultured Neonatal Myocyte Hypetrophy Induced by AngⅡ in Rats
- 中国实验方剂学杂志 2008年14卷第5期页码：40-43
- 作者机构：
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- 基金信息：
  
  国家自然科学基金资助项目(No:30772693);教育部博士点专项基金资助项目(20050026004)
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- 纸质出版日期：2008
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苏敬泽, 农一兵, 温志浩, 等. 黄芪组分配伍对乳鼠肥大心肌细胞肌酸激酶同功酶mRNA表达的影响[J]. 中国实验方剂学杂志, 2008,14(5):40-43.

SU Jing-ze, NONG Yi-bing, WEN Zhi-hao, et al. Radix Astragali Affect mRNA Expression of Isoenzyme Creatine Kinase of Cultured Neonatal Myocyte Hypetrophy Induced by AngⅡ in Rats[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(5): 40-43.
苏敬泽, 农一兵, 温志浩, 等. 黄芪组分配伍对乳鼠肥大心肌细胞肌酸激酶同功酶mRNA表达的影响[J]. 中国实验方剂学杂志, 2008,14(5):40-43. DOI：

SU Jing-ze, NONG Yi-bing, WEN Zhi-hao, et al. Radix Astragali Affect mRNA Expression of Isoenzyme Creatine Kinase of Cultured Neonatal Myocyte Hypetrophy Induced by AngⅡ in Rats[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(5): 40-43. DOI：

摘要

目的:探讨黄芪组分(黄芪皂苷和黄芪多糖)对心肌细胞能量代谢的干预作用及其分子机制

为中药防治心衰提供实验依据。方法:体外培养的乳鼠心肌细胞

加血管紧张素Ⅱ(AngⅡ)造成肥大心肌细胞模型

黄芪组分配伍干预后

采用RT-PCR法测定肌酸激酶(CK)同功酶的mRNA表达。结果:1.AngⅡ作用24h后

CK-B亚基的表达增加而CK-M亚基的表达减少

48h该作用持续存在;2.黄芪不同组分及配伍干预48h

心肌细胞的CK-B亚基及CK-M亚基表达趋于正常。结论:黄芪组分及组分配伍可在分子水平上抑制AngⅡ对心肌细胞CK同功酶mRNA表达的影响

提示黄芪可能是通过对肥大心肌细胞能量代谢的干预作用预防心衰的发生发展。

Abstract

Objective:To observe whether Radix Astragali saponins and Astragali polysaccharides can effectively interfere with the cellular energy metabolism and its mechanism and to provide scientific evidence for the application of the Chinese herbs preventing and treating congestive heart failure.Methods:mRNA expression of isoenzyme creatine kinase(iso-CK) was investigated by means of RT-PCR

based on the study of the effect of Radix Astragali saponins and Astragali polysaccharides

through the cultured neonatal myocyte hypetrophy induced by AngⅡ.Results: 1.The expression of CK-B subunits was increased significantly while the expression of CK-M subunits was decreased by AngⅡ since the 24th hours

at 48th hours

the expression still stay the same level.2.The expression of iso-CK: Subunits in Radix Astragali saponins and Astragali polysaccharides influenced kept it at the normal level at 48th hours.Conclusion: The influence of AngⅡ on the mRNA expression of isoenzyme creatine kinase can be effectively inhibited by Radix Astragali saponins and Astragali polysaccharides.Radix Astragali can prevent the development of heart failure

the possible mechanism is Radix Astragali interferes the myocyte hypetrophy in terms of the cellular energy metabolism.

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