Objective:To investigate the anti-inflammatory and neuroprotective action of Guiling Pill(Fuling

Baizhu and Danggui

FBD) on ischemic-reperfusion induced brain injury.Methods: Mice were orally administered 37.5 mg·kg-1 supercritical fluid extract of FBD(FBD-CO2)

150 mg·kg-1 aqueous extract(FBD-H2O)

and 187.5 mg·kg-1 both(FBD) beforehand

twice daily for 3.5 d

and subjected to repetitive 10 min ischemia and 24 h reperfusion

then brain infarction was observed by 2

3

5-triphenyltertrazolium chloride(TTC) staining

the permeability of BBB was examined by Evans Blue dyeing

the total blood leukocytes and its three subpopulations were counted while cerebral myeloperoxidase(MPO) activity was detected

and levels in prostaglandin E2(PGE2) and nitric oxide(NO) were assayed by spectrophotometry.Results: Compared with the saline-pretreated mice

oral pretreatment with FBD or FBD-CO2 had not only obviously less Evans Blue influx

and brain infarction

but also recruitment of leukocytes

evidenced with maintained circulating neutrophils counts and low cerebral MPO activity;excessive cerebral prostaglandin E2(PGE2)

and nitric oxide(NO) production were markedly also restrained by FBD-CO2

rather than FBD-H2O.Conclusion: FBD exerted an anti-inflammatory and neuroprotective action on cerebral I/R

partially via inhibition of neutrophils recruitment and generation of NO and PGE2.Its anti-inflammatory effect was mainly related with FBD-CO2.