狼疮方含药血清对人近端肾小管上皮细胞过氧化物酶体增殖物激活受体γ表达及CD40、RANTES的影响

张亚杰; 阳晓; 陈伟英; 孔庆瑜; 董秀清; 余学清

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狼疮方含药血清对人近端肾小管上皮细胞过氧化物酶体增殖物激活受体γ表达及CD40、RANTES的影响

更新时间：2024-01-04

- 狼疮方含药血清对人近端肾小管上皮细胞过氧化物酶体增殖物激活受体γ表达及CD40、RANTES的影响
- Effect of Lupus Recipe Contained Serum on the Expression of PPARγ and TNFα with IFNγ Induced CD40 and RANTES on Renal Tubular Epithelial Cells
- 中国实验方剂学杂志 2008年14卷第4期页码：29-32
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金(30271668,30572290);教育部博士点专项基金(2002055805);广东省重点攻关项目(2003C30202)
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- 纸质出版日期：2008
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张亚杰, 阳晓, 陈伟英, 等. 狼疮方含药血清对人近端肾小管上皮细胞过氧化物酶体增殖物激活受体γ表达及CD40、RANTES的影响[J]. 中国实验方剂学杂志, 2008,14(4):29-32.

ZHANG Ya-jie, YANG Xiao, CHENG Wei-ying, et al. Effect of Lupus Recipe Contained Serum on the Expression of PPARγ and TNFα with IFNγ Induced CD40 and RANTES on Renal Tubular Epithelial Cells[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(4): 29-32.
张亚杰, 阳晓, 陈伟英, 等. 狼疮方含药血清对人近端肾小管上皮细胞过氧化物酶体增殖物激活受体γ表达及CD40、RANTES的影响[J]. 中国实验方剂学杂志, 2008,14(4):29-32. DOI：

ZHANG Ya-jie, YANG Xiao, CHENG Wei-ying, et al. Effect of Lupus Recipe Contained Serum on the Expression of PPARγ and TNFα with IFNγ Induced CD40 and RANTES on Renal Tubular Epithelial Cells[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(4): 29-32. DOI：

摘要

目的:观察狼疮方含药血清对人近端肾小管上皮细胞(HK-2)过氧化物酶体增殖物激活受体γ(PPARγ)及干扰素(INFγ)和肿瘤坏死因子(TNFα)诱导的白细胞分化抗原40(CD40)、受激活调节的正常T细胞表达和分泌因子(RANTES)表达的影响

探讨该复方体外抗炎效应及其可能机制。方法:制备狼疮方含药大鼠血清;HK-2细胞株体外培养

分组如下:(1)培养基对照组;(2)正常鼠血清对照组;(3)狼疮方含药血清组;(4)IFNγ+TNFα刺激组;(3)刺激+10%狼疮方血清组;(4)刺激+10%正常鼠血清对照组。采用免疫印迹(Western-blot)检测PPARγ蛋白表达

半定量逆转录多聚酶链反应(RT-PCR)检测CD40mRNA、RANTESmRNA表达水平。结果:(1)正常HK-2细胞基础量表达PPARγ;10%狼疮方含药血清作用下

PPARγ蛋白表达呈现时间依赖性先增高后降低的趋势。(2)正常HK-2细胞基础水平表达CD40、RANTES。IFNγ+TNFα刺激后

CD40和RANTES表达显著升高;10%狼疮方含药血清可显著抑制IFNγ+TNFα诱导的CD40和RANTES表达;正常鼠对照血清对CD40和RANTES表达无明显影响。结论:狼疮方含药血清可能通过激活PPARγ途径抑制人近端肾小管上皮细胞CD40和RANTES表达

从而发挥抗炎效应。

Abstract

Objective:To observe the effect of Lupus Recipe on the expression of PPARγ and TNFα with IFNγ induced CD40 mRNA and RANTES mRNA on renal tubular epithelial cells(HK-2).Methods:Rats weighted 180～230 g were randomly divided into control group and treated group.The treated group was treated with Lupus Recipe(10 g·kg-1·day-1) daily

and the control group was given the same volume of PBS.Rats were sacrificed at 7 days after treatment.Serum of rats were collected.HK-2 cells were cultured in DMEM/F12 with 10% fetal bovine serum in vitro and were divided into following groups: the DMEM medium control group.the normal rat serum group and the Lupus Recipe contained serum group

IFNγ(50 μg·mL-1)+TNFα(10 ng·mL-1) group

IFNγ(50 μg·mL-1)+TNFα(10 ng·mL-1) with 10% drug-contained serum groups and IFNγ(50 μg·mL-1)+TNFα(10 ng·mL-1) with 10% normal rat serum group

The expression of PPARγ protein was measured by Western-blotting analysis and the expression of CD40 mRNA and RANTES mRNA was measured by RT-PCR.Results:HK-2 cells expressed low levels of PPARγ

CD40 mRNA and RANTES mRNA.Drug-contained serum of Lupus Recipe can up-regulate the expression of PPARγ protein.Activation of HK-2 cells by TNFα and IFNγ resulted in an enhanced expression of CD40 mRNA and RANTES mRNA;The Drug-contained serum of Lupus Recipe can significantly blocked CD40 mRNA and RANTES mRNA expression in HK-2 cell line induced by IFNγ plus TNFα.Conclusions:Drug-contained serum of Lupus Recipe up-regulated the expression of PPARγ protein and decreased CD40 and RANTES mRNA expression induced by IFNγ plus TNFα in HK-2 cells.These immune modulation and anti-inflammation effects were partially mediated by PPARγ-dependent pathways and it could be one of the mechanisms of Lupus Recipe in preventing and treating LN.

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