LIU Li, LIU Qiang, LIU Nu-yun. Study on Extracting Technology for Leech in Shenkang Micro-pellet[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(4): 19-21.
LIU Li, LIU Qiang, LIU Nu-yun. Study on Extracting Technology for Leech in Shenkang Micro-pellet[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(4): 19-21.DOI:
Objective:To inspect the extracting technology for leech in Shenkang Micro-pellet.Methods:With anticoagulation bioactivity as main Index and extraction rate as secondary one
optimum extracting conditions for leech in Shenkang Micro-pellet were studied by the orthogonal design.Results:The order of the factors which affected extraction was: ethanol concentration>extraction time>solvent amount.And the ethanol concentration had significant influence. Conclusions:The optimum extracting conditions were six times quantity of 60% ethanol for 2 times at 60℃