Objective:To compare the contents of the main active components in Guanxin Ⅱ Decoctions(GX-Ⅱ) which were decocted with different ways.Methods:The five ingredients in the formula were decocted in 8 modes

ie

decocting singlely

decocting two in 4 different combinations

and decocting all ingredients together.The contents of the active components in different decoctions were determined by HPLC methods.Results:The predominant ingredient

Danshen Root

in this formula was decocted with other four ingredients(Szechwan Lovage Rhizoma

Safflower

Red

Peony Root and Rose Wood) respectively.The extract rates of salvianolic acid B in the above four decoctions were higher than that of Danshen Root decoction.And the extract rates of salvianolic acid B was the highest in the decoction that five ingredients in this formula were decocted together.The extract rates of paeoniflorin in decoctions were similar with that of salvianolic acid B.But the rates of ferulic acid and hydroxymethyl safflower yellow A were contrary to that of the other two active components.When Szechwan Lovage Rhizome or Rosewood was decocted with other

ingredients

the extract rates of feralic acid and hydroxy-methyl safflower yellow were low.Conclusions: The contents of the active components in the decoction(Ⅰ) which was decocted with all ingredients together were not equal to that of mixed decoctions(Ⅱ) which were decocted with each ingredient separately.The results of pharmacological tests showed that the effects of the decoction(Ⅰ) were better than that of the mixed decoctions(Ⅱ).