Objective:To establish the chromatographic fingerprints of Fu Zhi San by RP-HPLC.Methods:By RP-HPLC

acetonitrile and water including 0.1% Phosphoric acid were mobile phase with gradient elution

flow rate was 1.0 mL·min-1

the detection wavelength was 335 nm

and the temperature was 30℃.The fingerprints of different samples were compared with similarity evaluation software published by committee codex.Results:The RSD of relative peak areas and relative retention time of common peak in the test of precision

repeatability

and stability has not reached 2%

respectively.19 peaks were identified as the characteristic fingerprints.Average similarity was 0.996.Conclusion:This method is precise

practicable

well-repeated and simple.It can be used as the method to judge the quality of Fu Zhi San.