WANG Xu1, JIN Xiang-qun1, SUI Cheng2. Determination of Chlorogenic Acid in Shuangguan Ganning Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(3): 22-23.
WANG Xu1, JIN Xiang-qun1, SUI Cheng2. Determination of Chlorogenic Acid in Shuangguan Ganning Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2008, 14(3): 22-23.DOI:
Objective:To develop an HPLC method for determining chlorogenic acid in Shuangguan Ganning Granule.Methods: Diamonsil C18 column(250 mm×4.6 mm
5 μm)was used as the stationary phase
the mixture of acetonitrile-0.4% phosphoric acid(13∶87) was served as mobile phase
and its flow rate was 1.0 mL·min-1.The wavelength for detection was 327 nm.Results:The linearity range was(0.04~0.37) μg(r=(0.999 8).) The average recovery was 99.50%(RSD=0.84%).Conclusion: The method is simple