Objective:To investigate the influence of Huanglianjiedu Tang drug serum on the expression of TLRs and downstream signaling components in RAW264.7.Methods:RAW264.7 cell line was stimulated with Lipopolysaccharide and Poly(I:C)

respectively

then treated with the drug serum of Huanglianjiedu Tang at the same time

after 24 hours

collected the supernatant and measured the inflammatory factors such as TNF-α and IFN-β

observed the expression of TLR3

TLR4

MyD88

TRAF-6

TRAM and TRIF mRNA with real-time PCR

using western blotting method to analyze the expression of TLR3

TLR4 antibody

and using cell immumofluorescence method to analyze the expression of MyD88 and TRAF-6 antibody.Results:Huanglianjiedu Tang drug serum decreased the high expression of TLR4

MyD88

TRAF-6

TRAM and TRIF(vs LPS stimulated group

P<0.05 or P<0.01)

but it only weakened the expression of TRAM and TRIF when the cells were stimulated by Poly(I:C).Conclusion:Huanglianjiedu Tang blocked the high expression of TLR4

blocked both MyD88-dependent and MyD88-independent signaling pathways(mainly blocked the MyD88-independent signaling pathway)

inhibited the excess secrteion of related gene expression products such as TMF-α

IFN-β

showed TLR4-antagon-like actions.Huanglianjiedu Tang also effected the junction protein such as TRAM and TRIF directly

influenced the MyD88-independent signaling pathway of TLR3

as a result

it inhibited the excess secretion of IFN-β.