ZHOU Xuan, SONG Fen-Yun, ZHONG Zhao-jian. Determination of Rutin and Quercetin in Flos Sophorae with Thin Layer Chromatogram Scanner[J]. Chinese journal of experimental traditional medical formulae, 2006, 12(8): 14-15.
ZHOU Xuan, SONG Fen-Yun, ZHONG Zhao-jian. Determination of Rutin and Quercetin in Flos Sophorae with Thin Layer Chromatogram Scanner[J]. Chinese journal of experimental traditional medical formulae, 2006, 12(8): 14-15.DOI:
Objective:To develop a TLCS method for determination of rutin and quercetin in Flos sophorae.Method:A TLCS method was used.The developer was a micro-emulsion consisted of soldium dodecyl sulphate/n-C4H9OH/n-C6H14/H2O/formicacid(0.27∶0.63∶0.10∶3∶0.2) and the thin layer was polyamide film.The spots of rutin and quercetin were well separated and scanned at 370nm with reference wavelength of 550nm.Results:The linear ranges of calibration curves were 0.2~3.0μg for rutin
0.1~1.0μg for quercetin.The recoveries of rutin and quercetin were 98.8% and 97.1% respectively.Conclusion:A TLCS method has been developed for the simultaneous determination of rutin and quercetin in Flos sophorae.