WEI Hua-ling, YANG Qing, WEI Hong-yin. Determination of Quercetin in Hawthorn Leaves by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2006, 12(8): 10-11.
WEI Hua-ling, YANG Qing, WEI Hong-yin. Determination of Quercetin in Hawthorn Leaves by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2006, 12(8): 10-11.DOI:
Objective:To develop a HPLC method for determining the content of quercetin in hawthorn leaves.Methods:The Suntek ODS column with methanol-0.4% phosphoric acid(45∶55) as the mobile phase was used.The flow rate was 0.9mL/min and the detective wavelength was 360nm.Results:The linear range of calibration curve was 0.1624~1.624μg/mL
for the quercetin(r=0.9998).The average recovery was 97.9% and the RSD was 0.59%(n=6).Conclusion:The method is simple
and accurate with a good repeatability and can be used as quantitative analysis for this preparation.