Objective:To study the effects of YangYuTuJi(YYTJ)on cell proliferation of diabetic rats caused by streptozotocin(STZ)

and its effects on fibroblast proliferation and migration in vitro.Methods:SD male rats except for control group(C)were given 55mg/kg STZ by intraperitoneal

randomly divided into model group(M) and 3 diferent dose groups of YYTJ.A round skin of φ1.6cm was excised on the back of rats.The healing time and healing rate were observed.Proliferation cell nuclear antigen(PCNA)was studied by immuno-histochemistry assay.Fibroblasts from normal and wounded site of diabetic rat skin were cultured as normal fibroblast(NFB) and diabetic fibroblast(DFB).MTT assay was used for cell proliferation

and cell wound model in vitro for migration analysis.Results:Three YYTJ groups shorten the healing time of the wound of diabetic rats caused by STZ(P<0.01 respectively)

and increased the healing rate(P<0.05

P<0.01 respectively).From day 3 the PCNA expression elevated in YYTJ-H group

day 5 in YYTJ-M group and day 7 in YYTJ-L group(P<0.05 and P<0.01 respectively).In vitro study we found YYTJ at both 29.23μg/mL and 58.45μg/mL promoted DFB proliferation and its migration.Conclusion:It is possible that YYTJ accelerates wound healing by promoting cell proliferation and cell migration.