GUI Shuang-ying, ZHOU Ya-qiu, KE Zhong-cheng. Adsorption and Separation Properties of D Resin for Purification of Paeoniflorin[J]. Chinese journal of experimental traditional medical formulae, 2006, 12(2): 25-27.
GUI Shuang-ying, ZHOU Ya-qiu, KE Zhong-cheng. Adsorption and Separation Properties of D Resin for Purification of Paeoniflorin[J]. Chinese journal of experimental traditional medical formulae, 2006, 12(2): 25-27.DOI:
Objective:To study the separation effect of D101 macroporous resin for the purification of Paeoniflorin in Radix Paeonia.Methods:HPLC was used to analyze the content of Paeoniflorin
and the adsorption performance and the elution parameters were investigated.Results:The optimal separation conditions were as follows:the concentration of Paeoniflorin was 2.5mg/mL with a flow rate of 3.6BV/h
and 20% alcohol was used as eluant.The adsorption of Paeoniflorin was 32.2mg/g
and the elution ratio of Paeoniflorin was 91.25%
and the purity of Paeoniflorin reached 73.70%.Conclusion:D101 resin can be used to refine the Paeoniflorin in the extraction of Radix Paeonia.