TIAN Juan1, 2, WANG Zhi-min1, et al. Determination of Matrine and Oxymatrine in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2006, 12(2): 23-24.
TIAN Juan1, 2, WANG Zhi-min1, et al. Determination of Matrine and Oxymatrine in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2006, 12(2): 23-24.DOI:
Objective:To establish a high performance liquid chromatography(HPLC)method to determine the contents of matrine and oxymatrine in Sophora flavescens Ait.for selecting its suitable planting areas.Methods:A YWG-NH_2 was used as the solid phase
acetonitrile-3% phosphoric acid-ethand(80∶10∶10)as the mobile phase
with a detection wavelength at 220nm.Results:The calibration curves of matrine and oxymatrine were in good linearity over the rang of 0.050~0.061μg and 0.372~4.464μg
respectively.Conclusion:Sophora flavescens growing in Licheng
Zuoquan
Linfen in Shanxi province and Taibai in Shaanxi province contained higher oxymatrine than 3%.Content differences of matrine were smaller in different growing places.