Object:To study the effects and mechanism of total flavone from the flowers of Ablemoschl Manihot L.Medic(Tfa) against myocardium injury induced by ischemia and reperfusion.Methods:The ischemia-reperfusion model was induced by after 30 min of coronary occlusion followed by 3.5 h of reperfusion in rats.The creatine phosphokinase(CPK) of serum

the malondialdehyde(MDA) and the superoxide dismutase(SOD) of myocardium were measured.The cell injure model in the cultured neonatal rat cardiomyocytes was induced by anoxia-reoxygenation and remove oxygen and glucose from medium

and the lactate dehydrogenase(LDH) was detected.Apoptosis was determined by terminal deoxy-nucleotidyl transferase-mediated dUTP-digoxigenin nick end-labeling(TUNEL) method and Bcl-2 expression determined by immunohistochemistry method.Results:The results showed that Tfa can decreased the MDA contents in myocardium and the production of CPK in serum

improved the SOD activitie;also decreased the number of apoptotic cells and enhanced the Bcl-2 expression in injured rat myocardium by ischemia/reperfusion.Tfa showed it can reduced the release of LDH from injured cardiomyocytes.Conclusion:Tfa showed the protective effect against myocardial ischemia and reperfusion injured which might be resist the damaging of oxygen free radical(OFR) and inhibited cardiomyocyte apoptosis.