Objective:To measure the contents of aconitine alkaloids in Tongmai-Sini-Tang

and its regularity of vary with decoction time.Methods:A RP-HPLC method was used.The chromatographic conditions are listed below:Hypersil-BDS column(250mm×4.6mm

5μm)

a mobile phase composed of methanol-water-chloroform-triethylamine(100∶50∶3∶0.15)

a flow rate of 1 mL·min-1 and wavelenghts-234nm

column temperature was 30℃.Results:The linear range of aconitine was(0.0025～0.0800)μg(r=0.9997)

mesaconitine was(0.0028～0.0880)μg(r=0.9996)

and hypaconitine was(0.0023～0.0760)μg(r=0.9996).Conclusion:while decocting medicinal herbs except from major herb in the formulation

other herbs were effective on the contents of aconitine alkaloids.the compositions of aconitine and hypaconitine would disappear after decocting medicinal herbs for 70 minutes

but the mesaconitine disappear only half.