Objective:To study the inducing effect of Jianpi Bushen Formulation(JPBS)on apoptosis

the levels of mitochondrial membrane potential(MMP)and endocellular reactive oxygen species(ROS)in MGC-803 cells.Methods:Apoptosis induced by JPBS-containing serum.MMP and ROS in MGC-803 cells was detected using flow cytometry(FCM)(Annexin V

rhodamine 123 and dihydrorhodamine 123 were used

respectively).Results:After 36 hours of treatment

the apoptosis of the drug-treated groups was obviously higher than that of the control group

and the drug action showed significant time-effect relationhip (P<0.05);The endocellular ROS level of JPBS group maintained substantially constant

and the level of JPBS+DDP group was lower than that of the serum control group and JPBS group(P<0.05)after 36 hours of treatment;and the MMP level of JPBS group

DDP group and JPBS+DDP group reduced gradually with extended action duration.Conclusion:Apoptosis induced by JPBS in MGC-803 cells may show time-dependent

the MMP level may be reduced and the endocellular ROS level may keep constant;and

there were synergetic effect between JPBS and DDR.