高效液相色谱法测定藿香正气丸(大蜜丸)中厚朴酚、和厚朴酚含量

赵现红1; 李向日1; 袁永生2; 周玉新2

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高效液相色谱法测定藿香正气丸(大蜜丸)中厚朴酚、和厚朴酚含量

更新时间：2024-01-04

- 高效液相色谱法测定藿香正气丸(大蜜丸)中厚朴酚、和厚朴酚含量
- HPLC Method for the Quantitative Determination of Magnolol and Honokiol in the Huoxiang Zhengqi Wan
- 中国实验方剂学杂志 2005年11卷第2期页码：13-15
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- 纸质出版日期：2005
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赵现红1, 李向日1, 袁永生2, 等. 高效液相色谱法测定藿香正气丸(大蜜丸)中厚朴酚、和厚朴酚含量[J]. 中国实验方剂学杂志, 2005,11(2):13-15.

ZHAO Xian-hong1, LI Xiang-ri1, YUAN Yong-sheng2, et al. HPLC Method for the Quantitative Determination of Magnolol and Honokiol in the Huoxiang Zhengqi Wan[J]. Chinese journal of experimental traditional medical formulae, 2005, 11(2): 13-15.
赵现红1, 李向日1, 袁永生2, 等. 高效液相色谱法测定藿香正气丸(大蜜丸)中厚朴酚、和厚朴酚含量[J]. 中国实验方剂学杂志, 2005,11(2):13-15. DOI：

ZHAO Xian-hong1, LI Xiang-ri1, YUAN Yong-sheng2, et al. HPLC Method for the Quantitative Determination of Magnolol and Honokiol in the Huoxiang Zhengqi Wan[J]. Chinese journal of experimental traditional medical formulae, 2005, 11(2): 13-15. DOI：

摘要

目的:建立藿香正气丸(大蜜丸)中厚朴酚、和厚朴酚的含量测定方法。方法:采用高效液相色谱法

以ZORBAX SB-C18色谱柱(5μm

4.6mm×150mm

SN:USCL012593)

乙腈-水(68∶32)为流动相

流速为1.0mL/min

检测波长为294nm

室温25℃。结果:厚朴酚在0.18μg～3.5μg范围内呈良好的线性关系

该方法平均回收率为98.5%

RSD为1.5%；和厚朴酚在0.11μg～2.2μg范围内呈良好的线性关系

该方法平均回收率为101.8%

RSD为2.7%。结论:本方法简单、灵敏度高

可用于藿香正气丸(大蜜丸)中厚朴酚、和厚朴酚的含量测定。

Abstract

Objective:To establish a method for determining Magnolol and Honokiol in the Huoxiang Zhengqi Wan. Method:An HPLC method was designed

which was performed under the following conditions:Zorbax sb-C18 column (4.6mm×150mm

5μm) as the chromatographic column

acetonitrile-water(68∶32) as the mobile phase at a flow velocity of 1.0mL/min

and the wavelength of 294nm as the detecting wavelength. Results:The calibration curves were linear in the range of 0.18 μg～3.5 μg magnolol and 0.11 μg～2.2 μg honokiol. The RSD was 1.5%(n=5) and 2.7%(n=5)

and the average recovery was 98.5% and 101.8%

respectively. Conclusion:This HPLC method is easy and accurate

and can be used as one of the methods for controlling the quality of the Huoxiang Zhengqi Wan.

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