当归芍药散精简方对小鼠学习记忆障碍改善作用的物质基础与作用机理研究

王鹏; 余伯阳; 林志宏; 朱丹妮; 严永清

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当归芍药散精简方对小鼠学习记忆障碍改善作用的物质基础与作用机理研究

更新时间：2024-01-04

- 当归芍药散精简方对小鼠学习记忆障碍改善作用的物质基础与作用机理研究
- Study on Basis and Mechanism of Action of Optimized Danggui Shaoyao San in the Improvement Effect on Learning and Memory Impairment in Mice
- 中国实验方剂学杂志 2004年10卷第2期页码：18-21
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家新药基金资助项目(96-901-05-38);国家自然科学基金资助项目(30271604)
- DOI：
  中图分类号：
- 纸质出版日期：2004
- 稿件说明：
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王鹏, 余伯阳, 林志宏, 等. 当归芍药散精简方对小鼠学习记忆障碍改善作用的物质基础与作用机理研究[J]. 中国实验方剂学杂志, 2004,10(2):18-21.

Study on Basis and Mechanism of Action of Optimized Danggui Shaoyao San in the Improvement Effect on Learning and Memory Impairment in Mice[J]. Chinese journal of experimental traditional medical formulae, 2004, 10(2): 18-21.
王鹏, 余伯阳, 林志宏, 等. 当归芍药散精简方对小鼠学习记忆障碍改善作用的物质基础与作用机理研究[J]. 中国实验方剂学杂志, 2004,10(2):18-21. DOI：

Study on Basis and Mechanism of Action of Optimized Danggui Shaoyao San in the Improvement Effect on Learning and Memory Impairment in Mice[J]. Chinese journal of experimental traditional medical formulae, 2004, 10(2): 18-21. DOI：

摘要

目的:筛选当归芍药散精简方(FBD)抗血管性痴呆的有效部位;方法:(1)氢溴酸东莨菪碱与脑部反复缺血再灌注诱导小鼠记忆障碍

水迷宫实验和避暗实验检测小鼠游泳时间、避暗潜伏期与错误次数;(2)连二亚硫酸钠和谷氨酸钠诱导PC12细胞损伤

MTT法测定细胞活力;结果:(1)等生物剂量下

3个FBD化学部位组合[多糖+挥发油]、[多糖+挥发油+正丁醇部位]、[多糖+挥发油+水部位]可显著缩短小鼠游泳时间(P<0.05)

显著延长避暗潜伏期并减少5min内的错误次数(P<0.05～0.01);(2)10%浓度的3个部位组合含药血清均可改善PC12细胞损伤(P<0.1～0.05)

[多糖+挥发油]对小鼠记忆障碍的改善作用与抗PC12细胞损伤作用表现一致

均有显著性(P<0.05～0.01);结论:[多糖+挥发油]可能为FBD的防治血管性痴呆的有效部位。

Abstract

Objective:To distinguish the active fraction extracted from the optimized Danggui Shaoyao San(FBD)

which can prevent and treat vascular dementia.Method:(1) The dysmnesia model of learning and memory induced by scopolamine and ischemia-reperfusion was used. The ability of learning and memory was measured by step-through task and water maze task. (2) PC12 cell was injured by N`a2S2O4 and glutamine and the viability of cell was measured by MTT method.Results:At the same dosage of herbal medicine

the three compositions composed by four fractions extracted from FBD

[Polysaccharide+Volatile oil]

[Polysaccharide+Volatile oil+n-Butanol-fraction] and [Polysaccharide+Volatile oil +Water-fraction]

shortened swimming time obviously (P<0.05)

prolonged significantly step-through latency and decreased number of errors in 5 minutes (P<0.05～0.01). (2) 10% Serum containing FBD and the three compositions were manifested to increase viability of injured PC12 cell (P<0.1～0.05). [Polysaccharide+Volatile oil] worked most obviously (P<0.05～0.01) at behavior and cellular levels.Conclusion: [Polysaccharide+Volatile oil] might be the active fraction of optimized Danggui Shaoyao San for preventing and treating VD.

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