ZHANG Da-jun, WANG Zhao-hua, ZHAO Yu-lan, et al. Determination of Icariin in Qianliekangshu Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2003, 9(6): 10-11.
ZHANG Da-jun, WANG Zhao-hua, ZHAO Yu-lan, et al. Determination of Icariin in Qianliekangshu Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2003, 9(6): 10-11.DOI:
Objective:To determine the content of Icariin in Qianliekangshu Capsule by high performance liquid chromatography.Methods:The determination was conducted by HPLC using a Diamonsil-C18 column(5μm
4.6mm×150mm) and a mobile phase of Acetonitrile-water (30∶70).The detection wavelengh was 270nm.Results:Good linear relationship between area and amount was noted for 0.496~2.480μg of Icariin
with a correlation coefficient of 0.9997.The average recovery of Icariin was 97.20%
RSD was 1.88%.Conclusion:The established method is simple
accurate
sensitive and can be applied to the quality control of the preparation.