复方三根制剂对MDR细胞株K562/ADR和K562/VCR逆转作用的研究

谢长生; 周维顺; 冯正权; 郭勇; 吴良村

您当前的位置：

首页 >

文章列表页 >

复方三根制剂对MDR细胞株K562/ADR和K562/VCR逆转作用的研究

更新时间：2024-01-04

- 复方三根制剂对MDR细胞株K562/ADR和K562/VCR逆转作用的研究
- The Study on the Reversal Effects of Fufang Sangen Mixture in Multidrug Resistant Cell Lines K562/ADR and K562/VCR
- 中国实验方剂学杂志 2003年9卷第4期页码：26-30
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  中图分类号：
- 纸质出版日期：2003
- 稿件说明：
移动端阅览
谢长生, 周维顺, 冯正权, 等. 复方三根制剂对MDR细胞株K562/ADR和K562/VCR逆转作用的研究[J]. 中国实验方剂学杂志, 2003,9(4):26-30.

XIE Chang-shang, ZHOU Wei-shun, FANG Zheng-quan, et al. The Study on the Reversal Effects of Fufang Sangen Mixture in Multidrug Resistant Cell Lines K562/ADR and K562/VCR[J]. Chinese journal of experimental traditional medical formulae, 2003, 9(4): 26-30.
谢长生, 周维顺, 冯正权, 等. 复方三根制剂对MDR细胞株K562/ADR和K562/VCR逆转作用的研究[J]. 中国实验方剂学杂志, 2003,9(4):26-30. DOI：

XIE Chang-shang, ZHOU Wei-shun, FANG Zheng-quan, et al. The Study on the Reversal Effects of Fufang Sangen Mixture in Multidrug Resistant Cell Lines K562/ADR and K562/VCR[J]. Chinese journal of experimental traditional medical formulae, 2003, 9(4): 26-30. DOI：

摘要

目的:研究复方中药——复方三根制剂对多药耐药(MDR)细胞株的逆转作用。方法:应用MTT比色法

观察复方三根制剂对MDR细胞的逆转作用。运用流式细胞仪(FCM)

测定其对耐药细胞积聚和外排阿霉素(ADR)的影响

以及对耐药株细胞表达pgp的影响。结果:复方三根制剂可部分恢复K562/ADR和K562/VCR耐药细胞对ADR的敏感性

而对VCR抗药性的逆转作用不明显;可增加K562/ADR和K562/VCR耐药细胞内ADR的积聚

并对外排ADR有一定影响;可部分下调p-gp的表达。结论:复方三根制剂可部分逆转耐药细胞的抗药性。

Abstract

Obsjective To observe the reversal effect of Fufang Sangen Mixture(FFSG) in multidrug resistant(MDR)cell lines K562/ADR and K562/VCR.Methods We used MTT assay to observe the mixture's reversal effect.Then

we determined the effect of FFSG on intracellular adriamycin(ADR) accumulation

drug efflux and the expression of p-gp by flowcytometry(FCM).Results 10mg/ml

20mg/ml of FFSG can reverse the K562/ADR cell's resistance to adriamycin to some degree and 20mg/ml FFSG can also partly reverse the K562/VCR cell's resistance to adriamycin.10mg/ml

20mg/ml concentration of FFSG can enhance the intracellular adriamycin accumulation in K562/ADR and K562/VCR cell lines.FFSG also has the effect on the efflux of intracellular adriamycin and decreasing the expression of p-gp in MDR cell lines.Conclusion FFSG can partly reverse the resistance of MDR cell lines.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

暂无数据