FANG Su-ping, QIU Quan-ying, HAO Yu. Influence of Herbage Containing Serum of HuangLianJieDu Tang on LPS or TNF-α Induced Neutrophil-Endothelium Cells Adhesion[J]. Chinese journal of experimental traditional medical formulae, 2001, 7(2): 31-33.
FANG Su-ping, QIU Quan-ying, HAO Yu. Influence of Herbage Containing Serum of HuangLianJieDu Tang on LPS or TNF-α Induced Neutrophil-Endothelium Cells Adhesion[J]. Chinese journal of experimental traditional medical formulae, 2001, 7(2): 31-33.DOI:
Objects:To investigate the influence of herbage containing serum of HuangLianJieDu Tang(HLJDT)on LPS or TNF-α induced neutrophil-endothelium adhesion and its anti-inflammation mechanism.Methods:Based on the model of Human umbilical vein endothelial cell(HUVEC)
Rose Bengal Stain was used to study the influence of herbage containing serum of HLJDT on LPS or TNF-α induced neutrophil-endothelium adhesion by pretreated HUVEC4h、12h、24h.Results:The results showed the seram of 1.5h group can decrease cells adhesion by pretreated HUVEC with serum only
but all serum can decrease LPS/INF-α induced cells adhesion by pretreated HUVEC with serum and LPS or TNF-α.Conclusion:The herbage containing serum of HLJDT can decrease the neutrophil-endothelium cells adhesion
inhabiting neutrophil transude from vessel
which may be one of its anti-inflammation mechanisms.