In order to observe Huang Lian Jie Du Decoction (HLJDD)'s anti-inflammation mechanism and the best time to collect its herbage-containing serum from rats

human umbilical vein endothelial cells (HUVEC) cultured in vitro and protein staining was used to study the effect of serum on neutrophil-endothelial cell adhesion by treated with HUVEC for 4h、12h、24h

the serum was collected on 0.5h 1.0h 1.5h 2.0h 2.5h after treating with HLJDD (one dose once a day while another dose twice a day for three days).The results showed that the herbage-containing serum could decrease the cell adhesion in some degree with time-effect relationship.Compared to the control group

the best time to collect its serum was 1.5h around by dosed twice a day for three days.The HLJDD herbage-containing serum could decrease the neutrophil-endothelial cell adhesion

and inhibit neutrophil transuding from vessel

which might be one of HLJDD's anti-inflammation mechanisms.