WEI Feng-ling, SHAN Guang-xuan, ZHU Li-ping, et al. Determination Method of Anthraquinonoids in Radix et Rhizoma Rhei[J]. Chinese journal of experimental traditional medical formulae, 2002, 8(1): 14-15.
WEI Feng-ling, SHAN Guang-xuan, ZHU Li-ping, et al. Determination Method of Anthraquinonoids in Radix et Rhizoma Rhei[J]. Chinese journal of experimental traditional medical formulae, 2002, 8(1): 14-15.DOI:
Objective:To select the optimum extracted method of anthraquinonoids from radix et rhizoma Rhei by RP-HPLC.Methods:The main factors effecting the total anthraquinonoids
such as hydrolysis time
concentration and volume of acid
extraction times and volume of chloroform were examined by uniform design mthod.The data were calculated by UROS system.Results:The optimum extracted conditions:hydrolysis with 1mol/L H2SO4 10ml for 9min
extracted by 70ml chloroform for two times.Conclusion:This method was validated to show reliable and reproducible.