LIU Xing-chang, YANG Song-song, WANG Sheng-hui. Determinatin of Paeoniflorin in Puqi Gongtai Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2002, 8(3): 9-10.
LIU Xing-chang, YANG Song-song, WANG Sheng-hui. Determinatin of Paeoniflorin in Puqi Gongtai Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2002, 8(3): 9-10.DOI:
Objective:To determine the contents of Paeoniflorin in Puqi Gongtai granules by high performance liquid chromatography.Methods:The method used Zorbax-C18 column(5μm
4.6mmid×250mm)
acetonitrile:water:36% acetate acid(75∶425∶0.4) as the mobile phase.The detection wavelength was set at 230nm.Results:The method of linear range was 0.02~0.1μg
r=0.9998.The average recovery of the method was 98.84%
RSD =1.84%.Conclusion:The method to determine the contents of Paeoniflorin in Puqi Gongtai granules is simple
reliable
accurate and can be applied to the quality control of the preparation.