Objective The protective effects of drug-containing serum of Yi-Xin-Kang capsule

a traditional Chinese medicinal prescription

and the individual drugs of the prescription

Danshen and Shanzha

on H+-ATP ase activity of rat myocardial mitochondria injured by oxygen stress were studied.Method Myocardial mitochondria were incubated with oxygen stress injury system(Fe2+/VitC)for 30 min

and then the activity of H+-ATPase was determined. Results Oxygen stress significantly decreased the activity of H+-ATPase of myocardial mitochondria. Treatment with the drug-containing serum of H303 or the individual drugs of Danshen and Shanzha significantly protected the activities of the enzyme. The significant protective effects were observed in lower doses(100 and 200mg/kg) of H303-treated groups and large doses (600mg/kg)of Dan-Shen or Shan-Zha-treated groups. Conclusion H303-containing serum protects the activity of H+-ATPase

a key-enzyme of myocardial mitochondria respiratory chain

injured by oxygen stress

in which Dan-Shen and Shan-Zha may contribute to the effect of H303.