To investigate the protective effects of Xuefu Zhuyu (XEZY) decoction on the vascular endothelial cells injured by rabbit blood stasis syndrome (BSS) serum and its effects of improvement on the endocrine dysfunction

we used the 4 different kinds of rabbit serum (normal

BSS with absorbed XFZY

hyperlipemic) to cultured rabbit vascular endothelial cells in vitro. The concentrations of endothelin (ET)

nitric oxide (NO) and the activity of tissue-type plasminogen activator (t-PA)

plasminogen activator inhibitor (PAI)

antithrombin Ⅲ(AT-Ⅲ)in cultured supernatant were detected at 24 hour

respectively. Results show the levels of ET

NO and the activity of PAI

except for t-PA

increased significantly in the BSS serum group

as compared with normal serum group. No significant differences of these vaso-active substance levels in cultured supernatant between normal and BSS with XFZY decoction. These results indicate that XFZY decoction can improve the endocrine dysfunction of the endothelial cells and protect cultured endothelial cells injured by rabbit BSS serum in vitro.