TAN Xiao-mei, DAI Kai-jin, LIU Huan-huam, et al. Determination of Baicalin in Pill by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2001, 7(4): 4-5.
TAN Xiao-mei, DAI Kai-jin, LIU Huan-huam, et al. Determination of Baicalin in Pill by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2001, 7(4): 4-5.DOI:
The quantitative method of baicalin in gegen-qinlian pill was reported.The HPLC conditions as follows:C18 Column(250×4mm
10μm)
in room temperature
CH3OH∶0.2%H3PO4=48∶52 as mobile phase
flow rate in 1.0ml/min
detected wave length at 280nm
and the number of theoretical plates calculated by baicalin peak was not less than 3000.The average recovery and relative standard deviation were 100.8% and 1.075%(n=4)