WU Zhao-hui, TAN Xiao-mei, LUO Jia-bo. Determination of Puerarin in Gegen Qinlian Weiwan by HPLC[J]. Chinese journal of experimental traditional medical formulae, 1999, 5(6): 10-12.
WU Zhao-hui, TAN Xiao-mei, LUO Jia-bo. Determination of Puerarin in Gegen Qinlian Weiwan by HPLC[J]. Chinese journal of experimental traditional medical formulae, 1999, 5(6): 10-12.DOI:
Chromatograhic conditions were:ODS column;column temperature
room temperature;mobile phase
CH3OH-CH3CN-H2O(12∶8∶80);flow rate
0.8ml/min;detected at 250nm.The contents of Puerarin were calculated by external standard method.The number of theoretical plates calculated for Puerarin peak was no less than 2300.The standard curve was linear in the concentration range of 20~90μg/ml
and the correlation coefficient was 0.9999.The average recovery and the relative standard deviation were 98.74% and 2.06%(n=4)respectively.