SONG Hong-jie, QUAN Shan-cong, HU Jin-hong. Determination of Glycyrrhizin in Weiwanshu Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2000, 6(1): 5-7.
SONG Hong-jie, QUAN Shan-cong, HU Jin-hong. Determination of Glycyrrhizin in Weiwanshu Granule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2000, 6(1): 5-7.DOI:
The content of glycyrrhizin in weiwanshu granule was determined by high performance liquid chromatography.Chromatographic condition were as follows:internal standard:n-butyl p-hydroxybenzoate;mobile phase:methanol∶water∶36% acetic acid (65∶28∶7);column size:Shim-pack CLC-ODS(6.0mm×150mm);flow rate:1.0ml/min and detection wavelength:254nm.The method was linear within the range of 18~300μg/ml(r=0.9999
n=5).The average recovery
RSD
RSD of within-day and day-to-day were 98.35%
3.39%(n=9)
1.63% and 3.35%
respectively.This method was sensitive and highly reproducible.