ZHU Bing-hui1, LIANG Yi-ying1, MO Jin-yuan2. Determination of Icariin in Syrup by HPLC[J]. Chinese journal of experimental traditional medical formulae, 1999, 5(6): 8-10.
ZHU Bing-hui1, LIANG Yi-ying1, MO Jin-yuan2. Determination of Icariin in Syrup by HPLC[J]. Chinese journal of experimental traditional medical formulae, 1999, 5(6): 8-10.DOI:
The quantitative method of icariin in Anshenbunao syrup was reported.The HPLC conditions were:column of Hypersil ODS-2(250mm×4.6mm
5μm)
column temperature at 30℃
flow rate at lml/min
detected wavelength at 270nm
methanol-water(600∶400
v/v)as mobile phase.The theoretical plates of icariin were not lower than 6000.The average recovery and relative standard deviation(RSD) were 99.6% and 1.3%(n=5 )respectively.