东风桔总生物碱提取工艺优化

黄永昌; 尹永芹; 汤文伟; 张慧; 谌江城; 沈志滨

doi:10.11653/j.cnki.syfix.2014070040

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东风桔总生物碱提取工艺优化

更新时间：2024-01-04

- 东风桔总生物碱提取工艺优化
- Optimization of Extraction Process of Total Alkaloids in
- 中国实验方剂学杂志 2014年20卷第7期页码：40-42
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81202885）;国家“十二五”重大新药创制项目（2011zx09102-007-03）;广东省科技计划项目（2010B0307000444）;广州市科技计划项目（12A062131631）;广东省教育厅高层次人才项目（2010）
- DOI：10.11653/j.cnki.syfix.2014070040
  中图分类号：
- 纸质出版日期：2014
- 稿件说明：
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黄永昌, 尹永芹, 汤文伟, 等. 东风桔总生物碱提取工艺优化[J]. 中国实验方剂学杂志, 2014,20(7):40-42.

HUANG Yong-chang, YIN Yong-qin, TANG Wen-wei, et al. Optimization of Extraction Process of Total Alkaloids in [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 40-42.
黄永昌, 尹永芹, 汤文伟, 等. 东风桔总生物碱提取工艺优化[J]. 中国实验方剂学杂志, 2014,20(7):40-42. DOI： 10.11653/j.cnki.syfix.2014070040.

HUANG Yong-chang, YIN Yong-qin, TANG Wen-wei, et al. Optimization of Extraction Process of Total Alkaloids in [J]. Chinese journal of experimental traditional medical formulae, 2014, 20(7): 40-42. DOI： 10.11653/j.cnki.syfix.2014070040.

摘要

目的：建立东风桔总生物碱的含量测定方法并优选其提取工艺。方法：采用酸性染料比色法测定东风桔总生物碱含量，选择Buxifoliadine-B为指标成分，检测波长410 nm。以总生物碱提取量为指标，通过正交试验考察乙醇体积分数、乙醇用量、回流时间及提取次数对提取工艺的影响。结果：Buxifoliadine-B含量测定的线性范围0.034~0.34 g·L-1，加样回收率（99.16±1.81）%。最佳提取工艺为加10倍量80%乙醇回流提取2次，每次1.5 h；总生物碱提取量4.88 mg·g-1。结论：建立的东风桔总生物碱含量测定方法稳定可靠，优选的提取工艺方法合理可行。

Abstract

Objective: To establish a determination for total alkaloids in Atalantia buxifolia and optimize its extraction process. Method: The content of total alkaloids was determined by acid dye-colorimetry

taking Buxifoliadine-B as index ingredient

detection wavelength was set at 410 nm.Effects of ethanol concentration

ethanol volume

extracting time and times on extraction amount of total alkaloids were investigated by orthogonal test. Result: The content of Buxifoliadine-B had a good linear relationship within the range of 0.034-0.34 g·L-1

recovery was (99.16±1.81)%.Optimum extraction process was reflux extracted twice with 10 times the amount of 80% ethanol for 1.5 h per time;Under these conditions

extraction amount of total alkaloids was 4.88 mg·g-1. Conclusion: This determination for total alkaloids in A. buxifolia and optimized extraction process were reasonable and practicable.

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