Objective: To explore application feasibility of refractometry in purification technology of macroporous resin. Method: Taking extract of Citrus aurantium as research object

active ingredients in it was refined by AB-8 macroporous resin

washed with water and 50% ethanol

interval sampled

refractive ratio of eluent was monitored on line and analyzed its regularity.At the same time

HPLC and UV spectrophotometric method was used to determine hesperidin as references

and to judge initiation point and terminal point during elution. Result: In purification process of active ingredients from C. aurantium

water elution terminal point 4 BV(refractive index 0) of which was the initiation point by 50% ethanol

9 BV(refractive index 16) of 50% ethanol elution was the terminal point. Conclusion: Refractometry was accurate

reliable

convenient

quickly in purification technology of active ingredients of TCM

it had a strong practical significance.