HPLC同时测定全蝎不同工艺提取物中5种核苷类化合物含量

田晓然; 付廷明; 郭立玮

doi:10.11653/syfj2013080013

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HPLC同时测定全蝎不同工艺提取物中5种核苷类化合物含量

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- HPLC同时测定全蝎不同工艺提取物中5种核苷类化合物含量
- HPLC Simultaneous Determination of Five Nucleoside Compounds in Extracts of by Different Extraction Technologies
- 中国实验方剂学杂志 2013年19卷第8期页码：13-16
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家"十二五"重大新药创制项目(2011ZX09401-308-8)
- DOI：10.11653/syfj2013080013
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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田晓然, 付廷明, 郭立玮. HPLC同时测定全蝎不同工艺提取物中5种核苷类化合物含量[J]. 中国实验方剂学杂志, 2013,19(8):13-16.

TIAN Xiao-ran, FU Ting-ming, GUO Li-wei. HPLC Simultaneous Determination of Five Nucleoside Compounds in Extracts of by Different Extraction Technologies[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 13-16.
田晓然, 付廷明, 郭立玮. HPLC同时测定全蝎不同工艺提取物中5种核苷类化合物含量[J]. 中国实验方剂学杂志, 2013,19(8):13-16. DOI： 10.11653/syfj2013080013.

TIAN Xiao-ran, FU Ting-ming, GUO Li-wei. HPLC Simultaneous Determination of Five Nucleoside Compounds in Extracts of by Different Extraction Technologies[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 13-16. DOI： 10.11653/syfj2013080013.

摘要

目的: 比较水煎煮、醇提取以及湿法超微粉碎3种提取工艺对全蝎中尿嘧啶、次黄嘌呤、黄嘌呤、鸟苷、腺苷5种核苷类成分含量的影响。方法: 全蝎经不同工艺提取

采用HPLC外标法测定提取物中5种核苷化合物含量。Agilent C18色谱柱(4.6 mm×250 mm

5 μm)

流动相0.07%冰乙酸-甲醇梯度洗脱

流速1.0 mL·min-1

检测波长254 nm

柱温35 ℃。结果: 尿嘧啶、次黄嘌呤、黄嘌呤、鸟苷、腺苷5种核苷化合物均在一定质量浓度范围内线性关系良好

r均>0.999 5

样品中5种核苷化合物的加样回收率98.9%～103.9%

RSD均<3%。结论: 该HPLC简便快速、灵敏度高、重复性好

可应用于同时测定尿嘧啶、次黄嘌呤、黄嘌呤、鸟苷、腺苷5种核苷化合物含量并比较不同全蝎提取物之间的差异。

Abstract

Objective: To compare effects of 3 extraction technologies (water boiling

alcohol extraction and wet ultrafine grinding) on the content of uracil

hypoxanthine

xanthine

guanosine and adenosine from Buthus martensii. Method: Samples of B. martensii were extracted with different technologies

the content of five nucleosides was determined by HPLC.Conditions were as followings:Aglient Zorbax C18 column (4.6 mm×250 mm

5 μm)

gradient eluted with 0.07% ice acetic acid-methanol as mobile phase

flow rate 1.0 mL·min-1

detection wavelength 254 nm and column temperature 35 ℃. Result: Uracil

hypoxanthine

xanthine

guanosine

adenosine had a good linear relationship in a certain range of concentration

r>0.999 5

the average recoveries of five nucleoside compounds in all samples were between 98.9%-103.9% with RSD<3%. Conclusion: This established HPLC was convenient and accurate with good repeatability

it could be used to determine simultaneously the content of five nucleosides(uracil

hypoxanthine

xanthine

guanosine

adenosine) and compare difference among extracts of B. martensii by different technologies.

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