聚酰胺树脂分离纯化忍冬藤叶中绿原酸的工艺优选

喻菁; 王小平; 陈俊

doi:10.11653/syfj2013080023

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聚酰胺树脂分离纯化忍冬藤叶中绿原酸的工艺优选

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- 聚酰胺树脂分离纯化忍冬藤叶中绿原酸的工艺优选
- Optimization of Separation and Purification Technology for Chlorogenic Acids in Leaves of with Polyamide Resin
- 中国实验方剂学杂志 2013年19卷第8期页码：23-26
- 作者机构：
- 作者简介：
- 基金信息：
  
  江西省卫生厅中医药科研基金课题(2009A035)
- DOI：10.11653/syfj2013080023
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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喻菁, 王小平, 陈俊. 聚酰胺树脂分离纯化忍冬藤叶中绿原酸的工艺优选[J]. 中国实验方剂学杂志, 2013,19(8):23-26.

YU Jing, WANG Xiao-ping, CHEN Jun. Optimization of Separation and Purification Technology for Chlorogenic Acids in Leaves of with Polyamide Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 23-26.
喻菁, 王小平, 陈俊. 聚酰胺树脂分离纯化忍冬藤叶中绿原酸的工艺优选[J]. 中国实验方剂学杂志, 2013,19(8):23-26. DOI： 10.11653/syfj2013080023.

YU Jing, WANG Xiao-ping, CHEN Jun. Optimization of Separation and Purification Technology for Chlorogenic Acids in Leaves of with Polyamide Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 23-26. DOI： 10.11653/syfj2013080023.

摘要

目的: 优选忍冬藤叶中绿原酸的分离纯化工艺条件。方法: 以绿原酸含量为指标

通过单因素试验考察聚酰胺树脂的静态和动态吸附、解吸性能

并考察上样量、水洗用量、洗脱剂种类和用量;以绿原酸纯度和保留率为指标

采用L9(34)正交试验考察洗脱流速、洗脱剂浓度和用量对忍冬藤叶中绿原酸的分离纯化工艺的影响。结果: 优选的分离纯化工艺为绿原酸上样量10 mg·g-1树脂

用2 BV水洗除杂

加3 BV 65%乙醇洗脱

洗脱流速0.4 BV·h-1

收集洗脱液。结论: 优选的工艺合理、稳定可行

可为工业化分离纯化绿原酸提供参考。

Abstract

Objective: To optimize separation and purification technology of chlorogenic acid in leaves of Lonicera japonica. Method: With the content of chlorogenic acid as index

static and dynamic absorption

desorption properties of polyamide resin were observed by single factor test

and sample amount

the amount of washing water

type and dosage of eluent were investigated;With purity and retention rate as index

effects of elution flow rate

the concentration and volume of eluent on separation and purification technology was investigated by orthogonal test. Result: Optimized separation and purification technology was as following:sample amount 10 mg·g-1 resin

washed impurity with 2 BV water

and eluated with 3 BV the amount of 65% ethanol

flow rate of 0.4 BV·h-1

collected eluent. Conclusion: This optimized technology was stable and practicable

it could provide reference for industrial separation and purification of chlorogenic acid.

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