Objective: To optimize extraction technology of total flavonoids from Camellia oleifera seeds. Method: With extraction temperature

extraction time

ethanol concentration and solid-liquid ratio as independent variables

Box-Behnken central composite design was used to establish mathematical model

response surface and contour were made with yield of total flavonoids as response value

response surface methodology was adopted to optimize extraction technology of total flavonoids from C. oleifera seeds. Result: Optimum extraction technology conditions were as followings:extraction temperature 66 ℃

ethanol concentration 70%

extracting time 1.3 h

solid-liquid ratio of 1:22.Under these conditions

yield of total flavonoids from C. oleifera seeds was 1.87%

which was close to the theoretical value of 1.89%. Conclusion: This optimized technology was stable and reliable

and it could provide reference for rational utilization of resources of C. oleifera.